Porcine RPE/Choroidal Explant Cultures

8Citations
Citations of this article
12Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch’s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

References Powered by Scopus

The retinal pigment epithelium in visual function

2230Citations
N/AReaders
Get full text

The parameters of the porcine eyeball

233Citations
N/AReaders
Get full text

Epithelial-mesenchymal transition and proliferation of retinal pigment epithelial cells initiated upon loss of cell-cell contact

205Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Retina in a dish: Cell cultures, retinal explants and animal models for common diseases of the retina

93Citations
N/AReaders
Get full text

Fucoidans as potential therapeutics for age-related macular degeneration—current evidence from in vitro research

22Citations
N/AReaders
Get full text

Basolateral activation with TLR agonists induces polarized cytokine release and reduces barrier function in RPE in vitro

13Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Klettner, A., & Miura, Y. (2019). Porcine RPE/Choroidal Explant Cultures. In Methods in Molecular Biology (Vol. 1834, pp. 109–118). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8669-9_8

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 4

67%

Researcher 2

33%

Readers' Discipline

Tooltip

Immunology and Microbiology 3

50%

Agricultural and Biological Sciences 1

17%

Medicine and Dentistry 1

17%

Biochemistry, Genetics and Molecular Bi... 1

17%

Save time finding and organizing research with Mendeley

Sign up for free