Structure-function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family

81Citations
Citations of this article
148Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Alcohol oxidases, including carbohydrate oxidases, have a long history of research that has generated fundamental biological understanding and biotechnological applications. Despite a long history of study, the galactose 6-oxidase/glyoxal oxidase family of mononuclear copper-radical oxidases, Auxiliary Activity Family 5 (AA5), is currently represented by only very few characterized members. Here we report the recombinant production and detailed structure-function analyses of two homologues from the phytopathogenic fungi Colletotrichum graminicola and C. gloeosporioides, CgrAlcOx and CglAlcOx, respectively, to explore the wider biocatalytic potential in AA5. EPR spectroscopy and crystallographic analysis confirm a common active-site structure vis-à-vis the archetypal galactose 6-oxidase from Fusarium graminearum. Strikingly, however, CgrAlcOx and CglAlcOx are essentially incapable of oxidizing galactose and galactosides, but instead efficiently catalyse the oxidation of diverse aliphatic alcohols. The results highlight the significant potential of prospecting the evolutionary diversity of AA5 to reveal novel enzyme specificities, thereby informing both biology and applications.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Yin, D. T., Urresti, S., Lafond, M., Johnston, E. M., Derikvand, F., Ciano, L., … Brumer, H. (2015). Structure-function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family. Nature Communications, 6. https://doi.org/10.1038/ncomms10197

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 53

60%

Researcher 24

27%

Professor / Associate Prof. 7

8%

Lecturer / Post doc 5

6%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 35

37%

Chemistry 29

31%

Agricultural and Biological Sciences 26

27%

Chemical Engineering 5

5%

Article Metrics

Tooltip
Mentions
References: 2
Social Media
Shares, Likes & Comments: 39

Save time finding and organizing research with Mendeley

Sign up for free