Two studies were carried out to evaluate indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) as internal markers obtained by 144-h incubation in situ and chromium oxide (Cr2O3), titanium dioxide (TiO2) and isolated, purified and enriched lignin (LIPE®) as external markers, and two total fecal collection schemes (3 or 5 days) to estimate digestibility in bovines. The experimental procedure was a randomized block design in a split plot arrangement. In the first trial, five crossbreed heifers fed sugar cane plus 1% of urea/ammonium sulfate and concentrate (1% of body weight) were used. There was no difference for dry matter digestibility when the collection schemes were compared (5 days vs 3 days). The nutrient digestibility estimated by iADF was underestimated and those estimated by iNDF, chromium oxide, titanium dioxide and LIPE®, did not differ from the digestibility calculated on total fecal collection. In the second trial, eight lactating cows fed corn silage and concentrate (4 kg/cow/day) were used. There was no difference for dry matter digestibility when the collection schemes were compared (5 days vs 3 days). The nutrient digestibility estimated by iNDF was overestimated and those estimated by iADF, chromium oxide, titanium dioxide and LIPE®, did not differ from the digestibility calculated on total fecal collection. Three days of fecal collection and the markers tested can safely be used to estimate digestibility. © 2009 Sociedade Brasileira de Zootecnia.
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de Andrade Ferreira, M., de Campos Valadares Filho, S., Marcondes, M. I., Paixão, M. L., Paulino, M. F., & Valadares, R. F. D. (2009). Avaliação de indicadores em estudos com ruminantes: Digestibilidade. Revista Brasileira de Zootecnia, 38(8), 1568–1573. https://doi.org/10.1590/S1516-35982009000800022