Electrochemical Determination of Isoniazid Using Gallic Acid Supported Reduced Graphene Oxide

Abstract

In this study, we report a facile and sensitive amperometric determination of isoniazid (INZ) content in urine samples based on gallic acid induced reduced graphene oxide (GA-RGO) modified glassy carbon electrode. The GA-RGO composite was prepared by simple sonication method with the aid of gallic acid. The GA-RGO composite was characterized by field emission scanning electron microscope, Raman spectroscopy, and X-ray diffraction pattern. The electrochemical behavior of GA-RGO modified electrode was evaluated by cyclic voltammetry (CV) towards the oxidation of INZ. The GA-RGO modified electrode showed excellent electrocatalytic activity and lower potential detection towards the sensing of INZ. The electroanalytical sensing of INZ exhibited acceptable linear response ranges from 0.05 to 783.1 μM with the limit of detection (LOD) of 0.01 μM. The GA-RGO modified electrode displayed good storage stability, excellent reproducibility toward the sensing of INZ. Moreover, the GA-RGO modified electrode was successfully applied to the determination of INZ content in urine samples, showed satisfactory recovery values for practical applications.