Sequencing and analysis of a Mycoplasma gallisepticum A5969 chromosome region containing the S10 and rrn23-5 operons

Abstract

A 20,115-nt region of the Mycoplasma gallisepticum A5959 genome was sequenced (GenBank accession no. AF036708). The region contains the rrn23-5 and S10 operons, the lactate dehydrogenase gene, and two open reading frames (ORF293 and ORF129/ORF171) coding for proteins of unknown function. The rrn23-5 operon includes genes for 23S and 5S rRNAs. The S10 operon includes genes for 20 ribosomal proteins, SecY transport protein, adenylate kinase, and methionine aminopeptidase, and lacks the infA-rpl36-rps13-rpoA-rpl17 genes found in the S10 operon of M. genitalium, M. pneumoniae, and Bacillus subtilis. The product of M. gallisepticum ldh is equally similar to the corresponding proteins of mycoplasmata and B. subtilis but contains only a part of the motif characteristic of the active center of lactate dehydrogenases. The chromosome region adjacent to the sequenced one contains uvrA, nrdE, nrdF, and ptsI.