T-cell acute lymphoblastic leukemia (T-ALL) is primarily a NOTCH1-driven disease, which represents approximately 15% of pediatric and 25% of adult newly diagnosed ALL cases. Gain-of-function NOTCH1 mutations are highly prevalent in T-ALL contributing to almost 60% of the cases. The protocol presented here describes a method for in vivo T-ALL transformation driven by the retroviral transduction of hematopoietic progenitors with oncogenic mutant forms NOTCH1 and subsequent transplant into recipient mice. This T-ALL transformation model allows the interaction between the leukemia cells and the bone marrow microenvironment, better recapitulating the physiological conditions that promote the development of the human disease, providing a versatile tool for both experimental therapeutics and functional genetics studies on T-ALL.
CITATION STYLE
Campagnari, A., & Belver, L. (2024). NOTCH1-Induced T-Cell Acute Lymphoblastic Leukemia In Vivo Models. In Methods in Molecular Biology (Vol. 2773, pp. 9–24). Humana Press Inc. https://doi.org/10.1007/978-1-0716-3714-2_2
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