Metabolomics Analysis of Aqueous Humor Based on Liquid Chromatography–Mass Spectrometry

Abstract

Aqueous humor (AH) is a transparent fluid that fills the anterior segment of the eye. The composition and level of metabolites in AH are important for understanding its physiology and changes caused by the occurrence of eye disease. A simple method for the preparation and analysis of AH samples was developed using the liquid chromatography–quadrupole time-of-flight mass spectrometry (LC–QTOF-MS) technique. The analyses were performed using two types of chromatography: reversed-phase liquid chromatography–mass spectrometry (LC-RP–MS) and hydrophilic interaction liquid chromatography–mass spectrometry (LC-HILIC–MS), in the sample prepared by one protocol.