Ischemic pre-conditioning has been shown to protect hearts against ischemia/reperfusion (I/R)-induced cardiac injury. However, it is not feasible in clinic. Many researchers have tried to introduce brief I/R in skeletal muscle to mimic cardiac ischemic pre-conditioning, called remote ischemia pre-conditioning (RIPC). Studies from our group and other groups have shown that RIPC induces the release of cytokines from skeletal muscle (myokines) for tissue protection. Myokines play a central role in repair, inflammatory, and immune responses after injury. Thus, the detailed protocol for RIPC might be useful for researchers to study mechanisms underlying RIPC-mediated tissue protection and crosstalk. Here, we describe a detailed RIPC protocol and show MG53 secretion after RIPC into the blood.
CITATION STYLE
Lee, K. E., Kim, J., Park, K. H., Ma, J., & Zhu, H. (2023). Remote Ischemic Pre-Conditioning (RIPC). In Methods in Molecular Biology (Vol. 2597, pp. 11–18). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2835-5_2
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