Cultivo in vitro da bananeira Prata Anã clone Gorutuba, em meio líquido, agitado e estacionário

Abstract

The aim of this study was to evaluate the in vitro cultivation of Prata Anã banana Gorutuba clone in different volumes of liquid medium in the stationary system and under agitation, compared with the traditional cultivation in semisolid medium. The experiment was conducted in a completely randomized design with factorial 5 x 2 + 1, with five volumes (10, 15, 20, 25 and 30 mL) of liquid medium and two cropping systems (agitation and stationary) and control (semisolid medium), with 4 replicates; each plot had a bottle with 4 explants. The number of shoots/explant observed by the control on the third subculture differs from some treatments in liquid medium. In the fourth subculture, the number of shoots/explant of the control differed from all treatments in liquid medium, and cultivation under agitation system favored a number of shoots/explant significantly higher than that obtained by the stationary system. In the elongation and rooting phases, the results obtained in liquid medium were better than the control. Comparing the two systems of cultivation in liquid medium, we noted greater growth of the plantlets, which remained under agitation with the volume of 30ml. The cultivation in semisolid medium is more efficient in the multiplication phase in vitro, and in the elongation and rooting phases, the best system to be adopted is the cultivation in liquid medium under agitation with 30 mL volume.