The polyglutamine diseases are caused by the expansion of CAG repeats. A key step in understanding the disease mechanisms, at the DNA and protein level, is the ability to produce recombinant proteins with specific length glutamine tracts which is a time-consuming first step in setting up in vitro systems to study the effects of polyglutamine expansion. Described here is a PCR-based method for the amplification of CAG repeats, which we used to incrementally extend CAG length by 3-5 repeats per cycle. This method could be translated into various contexts where amplification of repeating elements is necessary. © Springer Science+Business Media New York 2013.
CITATION STYLE
Robertson, A. L., & Bottomley, S. P. (2013). A method for the incremental expansion of polyglutamine repeats in recombinant proteins. Methods in Molecular Biology, 1017, 73–83. https://doi.org/10.1007/978-1-62703-438-8_5
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