DNA binding fluorescent proteins are a powerful tool for single-molecule visualization. In this chapter, we discuss a protocol for the synthesis of DNA binding fluorescent proteins and visualization of single DNA molecules. This chapter includes stepwise methods for molecular cloning, reversible staining, two-color staining, sequence-specific staining, and microscopic visualization of single DNA molecules in a microfluidic device. This content will be useful for DNA characterization using DNA binding fluorescent proteins and its visualization at the single-molecule level.
CITATION STYLE
Jin, X., Kim, Y. T., & Jo, K. (2023). DNA Visualization Using Fluorescent Proteins. In Methods in Molecular Biology (Vol. 2564, pp. 223–246). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2667-2_11
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