Matrix Application with ImagePrep

Abstract

In this chapter, matrix application to tissue samples with the ImagePrep device is explained. The operating principle of vibrational vaporization of the matrix solvent and gravitational deposition of the droplets is presented. An important feature of the device is the control of the deposition process with an optical sensor; the principle of this detection is described. The chapter includes an introduction to the simple user interface with the adjustment of wetness, incubation time, and matrix thickness as intuitive parameters. A brief introduction to method development possibilities for detailed control over the matrix deposition process is given.