Establishment and Characterization of SV40 T-Antigen Immortalized Porcine Muscle Satellite Cell

0Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.

Abstract

Muscle satellite cells (MuSCs) are crucial for muscle development and regeneration. The primary pig MuSCs (pMuSCs) is an ideal in vitro cell model for studying the pig’s muscle development and differentiation. However, the long-term in vitro culture of pMuSCs results in the gradual loss of their stemness, thereby limiting their application. To address this conundrum and maintain the normal function of pMuSCs during in vitro passaging, we generated an immortalized pMuSCs (SV40 T-pMuSCs) by stably expressing SV40 T-antigen (SV40 T) using a lentiviral-based vector system. The SV40 T-pMuSCs can be stably sub-cultured for over 40 generations in vitro. An evaluation of SV40 T-pMuSCs was conducted through immunofluorescence staining, quantitative real-time PCR, EdU assay, and SA-β-gal activity. Their proliferation capacity was similar to that of primary pMuSCs at passage 1, and while their differentiation potential was slightly decreased. SiRNA-mediated interference of SV40 T-antigen expression restored the differentiation capability of SV40 T-pMuSCs. Taken together, our results provide a valuable tool for studying pig skeletal muscle development and differentiation.

References Powered by Scopus

Satellite cell of skeletal muscle fibers.

2940Citations
N/AReaders
Get full text

Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases

2501Citations
N/AReaders
Get full text

T antigen is bound to a host protein in SY40-transformed cells [19]

1997Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Ni, M., He, J., Li, T., Zhao, G., Ji, Z., Ren, F., … Hou, L. (2024). Establishment and Characterization of SV40 T-Antigen Immortalized Porcine Muscle Satellite Cell. Cells, 13(8). https://doi.org/10.3390/cells13080703

Readers over time

‘2402468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 2

100%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 1

50%

Medicine and Dentistry 1

50%

Article Metrics

Tooltip
Mentions
Blog Mentions: 1
News Mentions: 1

Save time finding and organizing research with Mendeley

Sign up for free
0