Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays

51.1kCitations
Citations of this article
14.0kReaders
Mendeley users who have this article in their library.
Get full text

Abstract

A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. This method can therefore be used to measure cytotoxicity, proliferation or activation. The results can be read on a multiwell scanning spectrophotometer (ELISA reader) and show a high degree of precision. No washing steps are used in the assay. The main adavantages of the colorimetric assay are its rapidly and precision, and the lack of any radioisotope. We have used the assay to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis. © 1983.

References Powered by Scopus

Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition

1019Citations
N/AReaders
Get full text

Studies on succinate-tetrazolium reductase systems. III. Points of coupling of four different tetrazolium salts III. Points of coupling of four different tetrazolium salts

706Citations
N/AReaders
Get full text

The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes

215Citations
N/AReaders
Get full text

Cited by Powered by Scopus

New colorimetric cytotoxicity assay for anticancer-drug screening

9562Citations
N/AReaders
Get full text

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine

5873Citations
N/AReaders
Get full text

Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability

4671Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1–2), 55–63. https://doi.org/10.1016/0022-1759(83)90303-4

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 5896

72%

Researcher 1291

16%

Professor / Associate Prof. 689

8%

Lecturer / Post doc 307

4%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 2263

33%

Biochemistry, Genetics and Molecular Bi... 1999

29%

Chemistry 1703

25%

Pharmacology, Toxicology and Pharmaceut... 986

14%

Article Metrics

Tooltip
Mentions
Blog Mentions: 1
News Mentions: 10
References: 8
Social Media
Shares, Likes & Comments: 41

Save time finding and organizing research with Mendeley

Sign up for free