Characterization of recombinant β-amylases from Oryza sativa

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Abstract

Four putative β-amylase genes found in the Oryza sativa cDNA sequence database (KOME) were expressed in Escherichia coli. Recombinant proteins from two of these genes showed β-amylase activity. Similarly to β-amylases from other plants, the optimum pH of the recombinant rice β-amylases was about 5.5-6.0, but they exhibited inferior heat stability to soybean β-amylase.

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Oide, T. K., Hnishi, Y. O., & Orinouchi, S. H. (2011). Characterization of recombinant β-amylases from Oryza sativa. Bioscience, Biotechnology and Biochemistry, 75(4), 793–796. https://doi.org/10.1271/bbb.100872

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