Stress-Tolerant Viridibacillus arenosi Strain IHB B 7171 from Tea Rhizosphere as a Potential Broad-Spectrum Microbial Inoculant

Abstract

Viridibacillus arenosi strain IHB B 7171 identified based on 16S rRNA gene sequence produced colony forming units (cfu/ml) ranging from 3.3 × 104 to 1.2 × 1010 under pH 5–11, 2.2 × 102 to 1.4 × 1010 for temperature 5–40 °C, 2.4 × 102 to 1.1 × 1010 for PEG 6000 10–30%, 2.2 × 102 to 1.4 × 1010 for 2.5–10% NaCl, 3.1 × 103 to 1.7 × 109 for 2.5–7.5 mM CaCl2, 2.2 × 102 to 1.4 × 107 for 2.5–7.5 mM AlCl3, and 3.2 × 102 to 1.2 × 107 for 2.5–7.5 mM FeCl3. The activities of plant growth-promoting attributes with the increasing acidity, desiccation and salinity ranged from 408 to 101, 20 to 8, 14 to 5 µg/ml P-liberated from tri-calcium phosphate, aluminium phosphate and iron phosphate, 20–9% siderophore units, 14–4 µg/ml IAA and 190–16 α-ketobutyrate h/mg protein ACC-deaminase activity. Plant height, leaf number, and leaf weight on treatment with bacterial inoculum showed an increment of 9.5, 17.6, 54.5 and 31.0% in tea seedlings, respectively. The bacterium also enhanced plant height and yield by 10 and 13% in pea and 2.8 and 13.9% in wheat. The results exhibited stress-tolerance and plant growth-promoting activities by the strain under stressed growth-conditions with potential as a broad-spectrum plant growth-promoting rhizobacterium.