Fermentative Production of Lactobionic Acid by Burkholderia cepacia

  • Murakami H
  • Seko A
  • Azumi M
  • et al.
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Abstract

Lactobionic acid (LA) is derived from lactose (Lac) and has been reported to have several useful functions. LA has been supplied only as a reagent for study because it has been produced chemically in a small scale in Japan. We aimed to develop microbial production systems of LA and examined LA fermentation conditions. A three-day batch culture produced 200 g/L of LA. A ten-day fed-batch culture with periodic additions of Lac and CaCO3 accumulated 400 g/L of LA. There was no by-product and no loss of Lac, and the yield of LA-conversion was 100%. The addition of ethanol (75 % (v/v), final concentration) to culture supernatant resulted in recovery of 98 % of LA as precipitate.

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Murakami, H., Seko, A., Azumi, M., Ueshima, N., Yoshizumi, H., Nakano, H., & Kitahata, S. (2003). Fermentative Production of Lactobionic Acid by Burkholderia cepacia. Journal of Applied Glycoscience, 50(2), 117–120. https://doi.org/10.5458/jag.50.117

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