Antifungal Activity of Chitosan Based Biosynthesized Gold Nanoparticles Isolated from Portunus sanguinolentus Crab Shell

Abstract

Chitosan has become a prominent polymer attracting a lot of interest. The fact that this precious polymer may be recovered from shell waste created by the food industry adds to its worth. Because of its biological and physicochemical characteristics, chitosan has become a popular choice for biomedical applications. The different processes involved in the production of chitosan and chitosan Nano forms are briefly discussed in this article. We bring together for the first time, studies on diverse initiatives at green chitosan production, chitosan nanoparticles, and chitosan Nano composites. Chitosan aids in the production of more stable nanoparticles. The formation of nanoparticles was confirmed by UV-Visible spectroscopy, which detected a peak at 529 nm. Based on the interpretation of the spectroscopy in this study, the interaction between chitosan and generated gold nanoparticles (AuNPs) was studied and assessed, and it can be concluded that FT-IR spectroscopy was utilized to analyze all functional groups. XRD examination confirmed the presence of gold nanoparticles with crystalline nature, which were discovered to have an FCC lattice structure. Atomic force microscopy (AFM) is a technique for analyzing the surface of a rigid material all the way down to the level of the atom. AFM uses a mechanical probe to magnify surface features up to 100,000,000 times, and it produces 3-D images of the surface. The potency of the synthesized AuNPs against the selected fungi increases with increase in concentration ofAuNPs. Therefore, the antifungal investigation revealed that the synthesized AuNPs exhibited good antimicrobial activity.