An improved cowpea transformation method utilizing Agrobacterium-mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kanamycin and geneticin. The method was reproducible and led to the recovery of independent fertile transgenic plants in the greenhouse at a level of about one per cent of starting explants. A transgene encoding an insecticidal protein from Bacillus thuringiensis was used to demonstrate the efficacy of the system.
CITATION STYLE
Bett, B., Gollasch, S., Moore, A., Harding, R., & Higgins, T. J. V. (2019). An improved transformation system for cowpea (Vigna unguiculata l. Walp) via sonication and a kanamycin-geneticin selection regime. Frontiers in Plant Science, 10. https://doi.org/10.3389/fpls.2019.00219
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