Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 β-Cell Line: A Preclinical Model for Type 2 Diabetes

Abstract

In type 2 diabetes, prolonged dysregulation of signalling and β-cell metabolic control leads to β-cell dysfunction, and is increasingly associated with abnormal metabolic states which disrupt normal cellular physiology. Utilization of appropriate β-cell models enables a systematic approach to understand the impact of perturbations to the biological system. The BRIN-BD11 β-cell line is a useful, pre-clinical cell model for β-cell dysfunction associated with type 2 diabetes, among other metabolic disorders. The present chapter describes detection and analysis of β-cell dysfunction with respect to changes in bioenergetics and metabolism, generation of intracellular reactive oxygen species, and acute and chronic insulin secretion in the BRIN-BD11 cell line.