Abstract
This method allows you to isolate different subclass mononuclear cells, like B-cells, T cells, CD4+ and CD8+ T, from mouse spleen. By conjugating cells with specific antibodies and subsequently magnetic beads isolation, using the technique from Miltenyi, this allows a high purity.
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CITATION STYLE
APA
Weigmann, B. (2013). Cell Isolation of Spleen Mononuclear Cells. BIO-PROTOCOL, 3(9). https://doi.org/10.21769/bioprotoc.689
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