Pulmonary macrophages are heterogeneous. Distinct populations of resident tissue macrophages exist in the lung airspace and tissue compartments during homeostasis. During inflammation, these are joined by monocyte-derived recruited macrophages. Flow cytometry can be used to identify and purify lung macrophage subsets. Here, we describe methods for identifying and isolating macrophages from bronchoalveolar lavage and digested lung tissues from mouse and human. We also describe basic staining for flow cytometry analysis of different macrophage subsets.
CITATION STYLE
King, E. M., Hume, P. S., Janssen, W. J., & McCubbrey, A. L. (2022). Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung. In Methods in Molecular Biology (Vol. 2506, pp. 257–267). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2364-0_18
Mendeley helps you to discover research relevant for your work.