Promega

Abstract

This Standard Reference Material (SRM) is intended for use in establishing a reference scale for fluorescence intensity based upon Molecules of Equivalent Soluble Fluorophore (MESF) units [1–3]. This SRM is certified for the concentration of fluorescein with a certified purity in a borate buffer solution. The MESF scale is established for a particular set of experimental conditions by measuring the fluorescence intensity of known amounts of this SRM under a specific set of conditions (see “Instructions for Use”). A unit of SRM 1932 consists of three flamed sealed amber glass ampoules, each containing approximately 2.0 mL of fluorescein in an aqueous borate buffer.