High proportion of heterozygotes in microsatellite DNA Loci of wild clonal silver crucian carp, Carassius langsdorfii

Abstract

The silver crucian carp, Carassius langsdorfii has three reproductive characteristics: gynogenesis, polyploidy (triploid or tetraploid), and genetic homogeneity within a family. In natural water, the silver crucian carp populations consist of multiple clonal lines. In the present study, three microsatellite DNA loci were used to distinguish several clonal lines of the silver crucian carp sampled from natural water. Progeny and the maternal fish had the same genotype in the three loci. In 237 fish collected from the wild, nine alleles were observed in GF1*, sixteen alleles in GF17*, and nine alleles in GF29*. Ten genotypes were observed in GF1*, seventeen in GF17* and eight in GF29*. The proportion of heterozygotes was very high in each locus (1.000). Sixteen clonal lines were distinguished by the combined genotypes of three microsatellite loci. Two subtypes were also detected within the clonal line KOC-011.