Nowadays, high-throughput analysis of biological events is a great challenge which could take benefit of the recent development of microarray devices. The great potential of such technology is related to the availability of a chip bearing a large set of probes, stable and easy to obtain, and suitable for ligand-binding detection. Here, we describe a new method based on polypyrrole chemistry, allowing the covalent immobilization of peptides in a microarray format and on a gold surface compatible with the use of surface plasmon resonance. This technique is then illustrated by the detection and characterization of antibodies induced by hepatitis C virus and present in patients' serums. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
CITATION STYLE
Villiers, M. B., Cortès, S., Brakha, C., Marche, P., Roget, A., & Livache, T. (2009). Polypyrrole-peptide microarray for biomolecular interaction analysis by SPR imaging. Methods in Molecular Biology, 570, 317–328. https://doi.org/10.1007/978-1-60327-394-7_17
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