Nitric oxide modulates microvascular permeability

639Citations
Citations of this article
69Readers
Mendeley users who have this article in their library.
Get full text

Abstract

We recently demonstrated that inhibitors of nitric oxide (NO) production cause a dramatic increase in leukocyte adherence and emigration in postcapillary venules. The objective of this study was to assess whether inhibition of NO production leads to vascular protein leakage and increased microvascular permeability in feline small intestine and to determine whether adherent leukocytes contribute to these responses. Whereas N(G)-nitro-L- arginine methyl ester (L-NAME) produced fivefold increases in microvascular fluid and protein fluxes, capillary pressure remained unchanged. In some experiments, venous pressure was elevated and the microvascular reflection coefficient for total proteins (σ(d)) was estimated from lymph to plasma protein concentration ratio at high capillary filtration rates. L-NAME infusion increased 1 - σ(d) (permeability index) from a control value of 0.21 ± 0.02 to 0.41 ± 0.07. All of the L-NAME-induced microvascular alterations were completely reversed by nitroprusside. Some animals were pretreated with a monoclonal antibody (MoAb IB4) directed against the leukocyte adhesion glycoprotein complex CD11/CD18. MoAb IB4 did not prevent the initial rise in vascular protein leakage but greatly attenuated the later (30-60 min) phase of enhanced vascular protein leakage. Local intra-arterial infusion of the NO synthesis inhibitor L-NAME (0.025 μmol · ml-1 · min-1) produced a profound increase in leukocyte adhesion in postcapillary venules that was partly reversed by sodium nitroprusside administration (0.025 μmol · ml-1 · min-1). These results suggest that inhibition of NO production by vascular endothelium leads to a reversible increase in microvascular protein efflux that is mediated by both leukocyte-dependent and -independent mechanisms.

Cited by Powered by Scopus

Predominant role of endothelial nitric oxide synthase in vascular endothelial growth factor-induced angiogenesis and vascular permeability

867Citations
N/AReaders
Get full text

Vascular permeability factor/vascular endothelial growth factor and the significance of microvascular hyperpermeability in angiogenesis

661Citations
N/AReaders
Get full text

Nitric oxide and cardiac function

645Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Kubes, P., & Granger, D. N. (1992). Nitric oxide modulates microvascular permeability. American Journal of Physiology - Heart and Circulatory Physiology, 262(2 31-2). https://doi.org/10.1152/ajpheart.1992.262.2.h611

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 30

55%

Researcher 14

25%

Professor / Associate Prof. 9

16%

Lecturer / Post doc 2

4%

Readers' Discipline

Tooltip

Medicine and Dentistry 19

43%

Biochemistry, Genetics and Molecular Bi... 12

27%

Agricultural and Biological Sciences 7

16%

Engineering 6

14%

Save time finding and organizing research with Mendeley

Sign up for free