Pharmacological studies on crude plant drugs, Shikon and Tooki (III) Effect of topical application of ether extracts and Shiunko on inflammatory reaction

Abstract

Pharmacological effects of topical application of Shiunko, a main prescription of Shikon (Lithospermum Officinale Linne var. erythrorhizon Maximowicz), were studied in comparison with that of ether extracts of Shikon and Tooki. Ointment of Shikon ether extract (50 mg/site) inhibited markedly an increased vascular permeability and acute edema induced by histamine, serotonin, bromelain, anti-rat rabbit serum and heat. It also inhibited an increase of local cutaneous temperature induced by phlogistic stimulation (ultraviolet irradiation and heat). On the other hand, it accelerated wound healing in rats. The activity of Shikon ether extract on inflammatory reaction was the most potent in 0.2~0.1% of ointment concentration and was diminished in higher or lower concentrations. Tooki extract inhibited slightly an acute inflammatory reaction, but had no effect on increase of cutaneous temperature and wound healing in rats. Shiunko contains about 0.2% of Shikon and 0.04% of Tooki extract. The effect of Shiunko was similar to that of Shikon ether extract, but the activity was slightly more potent than that of Shikon. Shiunko inhibited rubor, calor and tumor in acute inflammatory reaction and promoted wound healing. Furthermore, it was reported that pigments of Shikon have antibiotic activity. The results suggest that Shiunko is an effective ointment for cutaneous injuries. © 1977, The Japanese Pharmacological Society. All rights reserved.