Anthocyanin and phenolic landscape of Syzygium cumini extracts via green extraction

Abstract

This study determined the anthocyanin and phenolic profile of Syzygium cumini bioactive compounds, including anthocyanins and other flavonoids, alongside diverse phenolic compounds. The study optimized a green extraction technique (ultrasound-assisted enzymatic extraction (UAEE)) to obtain anthocyanin-rich extract from the fruit pulp of S. cumini using the pectinase enzyme. UHPLC-LC/MS, FTIR, and SEM were used to profile the secondary metabolites, functional groups, and surface morphology. Two major anthocyanins, cyanidin and malvidin, and twenty-three non-anthocyanins, including gallic acid, naringenin, myricetin, and kaempferol, were identified in the enzymatic extract of S. cumini. A central-composite design was used to optimize the extraction, analyzing the effects of enzyme concentration (0.01–0.03 %), pH (1–3), and ultrasonication time (5–15 min) on total anthocyanin content (438.75 ± 29.81 mg C3G/100 g db), determining the optimal points (0.01 %, 2 pH and 10 mins). The optimized extract was further investigated for total phenolic content and antioxidant activities. The study utilized an economical approach to effectively extract maximum anthocyanins from S. cumini fruit for their potential applications as a biocolorant in food products, simultaneously establishing promising health potential through available literature.