In this chapter, we describe a stepwise protocol of microinjection. Using this method, antisense morpholinos, CRISPR-Cas9 ribonucleoprotein complexes, capped mRNA, and DNA can be delivered into fertilized zebrafish eggs to manipulate gene expression during development. This protocol can also be adapted for microinjection in other fish and amphibian species.
CITATION STYLE
Xin, Y., & Duan, C. (2018). Microinjection of antisense morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into zebrafish embryos. In Methods in Molecular Biology (Vol. 1742, pp. 205–211). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7665-2_18
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