Simultaneous determination of veterinary drugs in livestock products using dispersive and cartridge column solid-phase extraction by LC-MS/MS

Abstract

A method using dispersive and cartridge column solid-phase extraction (SPE) was developed for the simultaneous determination of veterinary drugs in livestock products by LC-MS/MS. The samples were extracted with 85% acetonitrile and cleaned up using dispersive and cartridge column SPE. Recoveries of 70 analytes fortified at the levels of 0.002 μg/g and 0.01 μg/g in cattle, swine and chicken muscles were examined. Among 70 analytes examined, 64 in cattle, 58 in swine and 49 in chicken satisfied the required Japanese validation guideline. The limit of quantitation values of the 70 analytes were 0.001 μg/g or 0.005 μg/g in three kinds of livestock products. This method is suitable to analyze 40 analytes in three kinds of livestock products at levels under the MRL.