Here we describe the use of synthetic genetic elements to improve the predictability and tunability of episomal protein production in Salmonella. We used a multi-pronged approach, in which a series of variable-strength synthetic promoters were combined with a synthetic transcriptional terminator, and plasmid copy number variation. This yielded a series of plasmids that drive uniform production of fluorescent and endogenous proteins, over a wide dynamic range. We describe several examples where this system is used to fine-tune constitutive expression in Salmonella, providing an efficient means to titrate out toxic effects of protein production.
CITATION STYLE
Cooper, K. G., Chong, A., Starr, T., Finn, C. E., & Steele-Mortimer, O. (2017). Predictable, tunable protein production in Salmonella for studying host-pathogen interactions. Frontiers in Cellular and Infection Microbiology, 7(NOV). https://doi.org/10.3389/fcimb.2017.00475
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