Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including "hard-to-transfect" cells. As a consequence of the integration of the recombinant lentiviral vector into the cellular genome, the transgene is stably maintained, and long-term producing cells are established. Here, we describe the current state of the art and give details for lab-scale production of lentiviral vectors as well as for infection and titration of the viral vectors.
CITATION STYLE
Gödecke, N., Hauser, H., & Wirth, D. (2024). Stable Expression by Lentiviral Transduction of Cells. Methods in Molecular Biology (Clifton, N.J.), 2810, 147–159. https://doi.org/10.1007/978-1-0716-3878-1_10
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