Assays for TE-031 (A-56268)in body fluids(I) microbiological assay

Abstract

To establish a bioassay for a new antibiotic, TE-031 (A-56268), in body fluids, various methods and conditions were studied. The most suitable assay used a disc plate method with Micrococcus luteus ATCC 9341 as test organism in heart infusion agar, pH 8.0. As standard solution of TE-031, consera for human serum, methanol phosphate buffer (methanol : 0.02M phosphate buffer, pH 7.4=4 : 1) and partly 1/15M phosphate buffer, pH 7.2 for urine, and methanol phosphate buffer for tissue were used. Samples were prepared by diluting the above standard solutions. The most active metabolite of TE-031, M-5, was also measured by the same bioassay. Its standard curve was almost the same as that of TE-031. The detectable range of TE-031 concentrations was 0.025~100 μg/ml (except 1/15M phosphate buffer : 0.2~100 μg/ml). There was good correlation between the bioassay and chemical assays. © 1988, Japanese Society of Chemotherapy. All rights reserved.