Identification of QTLs linked with watermelon fruit and seed traits using GBS-based high-resolution genetic mapping

14Citations
Citations of this article
7Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Watermelon is an economic horticultural crop of the Cucurbitaceae family. An in-depth genetic understanding of fruit and seed associated traits is important for the effective breeding of watermelon. In the present study, we conducted genotyping by sequencing (GBS)-based high-resolution genetic mapping and performed QTL mapping of twelve horticultural traits using segregated mapping populations (F2 and F2:3) derived from the breeding of two contrasting inbred parent lines. The developed genetic linkage map comprised of 6,164 SNP loci and 1,004 bins, which spanned a total genetic length of 1,261.13 cM with an average interval of 1.26 cM (329.31 kb) between adjacent whole-genome markers. Overall, a total of 34 QTLs were identified, and 16 QTLs exhibited moderate and major-effect QTLs; however, 25 were novel QTLs that were not identified in earlier studies. The mapped genetic loci of RC, RSP, and FFC traits were identified as closely overlapping with previously reported loci and exhibited delimited genetic intervals located on chromosomes 8, 6, and 4, respectively. The identified novel QTL (qfd2.1) of FD and QTL (qfl2.1) of FL were tightly localized with QTL (qfsi2.1) of FSI over chromosome 2, but a minor QTL (qfw3.2) positioned on chromosome 3 was consistent with previously published QTLs (qfd3.1, qfl3.1, and qfsi3.1) of fruit shape. A total of five minor-effect QTLs (qbcc2.1, qbce2.1, qbce2.2, qbcc5.1, and qbce5.1) identified in the novel genetic region which controlled the average Brix content of edible fruit. The seed width QTL (qsw10.1) was found to be localized with 20-seed weight QTL (q20swt10.1) on chromosome 10. The collected phenotypic dataset of two environmental experiments across the two years also revealed highly significant correlations among fruit and seed traits. Hence, we concluded that our GBS-based identified novel QTLs positions would expand powerful molecular breeding prospects for the genetic improvement of quality-related traits of watermelon.

References Powered by Scopus

Fast and accurate short read alignment with Burrows-Wheeler transform

35054Citations
N/AReaders
Get full text

ANNOVAR: Functional annotation of genetic variants from high-throughput sequencing data

10400Citations
N/AReaders
Get full text

R: A Language for Data Analysis and Graphics

9074Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Fine genetic mapping confers a major gene controlling leaf shape variation in watermelon

5Citations
N/AReaders
Get full text

Retrospective Genetic Analysis of Qualitative and Quantitative Traits in Sweet Watermelon (Citrullus lanatus var. lanatus): A Review

4Citations
N/AReaders
Get full text

Primary mapping of quantitative trait loci regulating multivariate horticultural phenotypes of watermelon (Citrullus lanatus L.)

4Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Liang, X., Gao, M., Amanullah, S., Guo, Y., Liu, X., Xu, H., … Luan, F. (2022). Identification of QTLs linked with watermelon fruit and seed traits using GBS-based high-resolution genetic mapping. Scientia Horticulturae, 303. https://doi.org/10.1016/j.scienta.2022.111237

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 3

75%

Researcher 1

25%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 4

80%

Biochemistry, Genetics and Molecular Bi... 1

20%

Save time finding and organizing research with Mendeley

Sign up for free