In vivo super-resolution imaging of transient retinal phototropism evoked by oblique light stimulation

Abstract

Rod-dominated transient retinal phototropism (TRP) has been observed in freshly isolated retinas, prom- ising a noninvasive biomarker for objective assessment of retinal physiology. However, in vivo mapping of TRP is challenging due to its subcellular signal magnitude and fast time course. We report here a virtually structured detection- based super-resolution ophthalmoscope to achieve subcel- lular spatial resolution and millisecond temporal resolution for in vivo imaging of TRP. Spatiotemporal properties of in vivo TRP were characterized corresponding to variable light intensity stimuli, confirming that TRP is tightly corre- lated with early stages of phototransduction. ©