Purification of Recombinant ADAMTSL2

Abstract

Recombinantly produced proteins are used in many biological disciplines. However, their purity and quality are vital for downstream applications used to determine their structure and functions. Several purification and detection strategies can be used in combination to obtain protein samples with homogeneity and structural conformity. Here we detail the protocols involved in the purification of ADAMTSL2 from mammalian cells. We also describe the protocols used to validate the purity of the protein samples.