Cellular chemotactic behaviors are typical examples of stochastic signal transduction in living cells that have been investigated in detail both experimentally and theoretically. In this chapter, we describe single-molecule kinetic analysis for stochastic signal transduction in chemotactic responses mediated by G protein-coupled chemoattractant receptors in order to give deeper understanding of the stochastic nature in chemotactic signaling processes. We also describe theoretical analysis of receptor-mediated chemotactic signaling, which reveals that noise generated in the transmembrane signaling by G protein-coupled chemoattractant receptors limits the precision of the gradient sensing. This suggests that receptor-G protein coupling and its modulation have an important role for improving the signal-to-noise ratio of chemotactic signals and thus cellular chemotaxis. Extending this beyond G protein signaling, combining single-molecule kinetic analysis with theoretical analysis offers a new tool in exploring the relationship between the kinetic properties of signaling molecules and their corresponding cellular responses in general. © Springer Science+Business Media B.V. 2011.
CITATION STYLE
Miyanaga, Y., & Ueda, M. (2011). Single-molecule kinetic analysis of stochastic signal transduction mediated by G-protein coupled chemoattractant receptors. In Cell Signaling Reactions: Single-Molecular Kinetic Analysis (pp. 33–57). Springer Netherlands. https://doi.org/10.1007/978-90-481-9864-1_2
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