CRISPR-based detection and recording technologies are gaining increasing attention in disease surveillance and prevention. In this chapter, we describe how our recent discovery of noncanonical crRNAs inspired the engineering of reprogrammed tracrRNAs and led to a powerful platform for multiplexed RNA detection. We provide detailed protocols regarding how to design reprogrammed tracrRNA and carry out assays in vitro and in vivo.
CITATION STYLE
Jiao, C., & Beisel, C. L. (2022). Reprogramming TracrRNAs for Multiplexed RNA Detection. In Methods in Molecular Biology (Vol. 2518, pp. 217–235). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2421-0_13
Mendeley helps you to discover research relevant for your work.