Laser Capture Microdissection for Analysis of Gene Expression in Formalin-Fixed Paraffin-Embedded Tissue

Abstract

A combination of laser capture microdissection and reverse transcriptase real-time quantitative PCR ­provides a powerful tool for the analysis of relative gene expression in archived tissue specimens. This chapter describes standard methodologies that can be used to determine the relative levels of gene expression in individual cells captured from formalin-fixed paraffin-embedded tissues.