Abstract
In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined. © Springer Science+Business Media New York 2014.
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CITATION STYLE
Bird, L. E., Rada, H., Flanagan, J., Diprose, J. M., Gilbert, R. J. C., & Owens, R. J. (2014). Application of In-FusionTM cloning for the parallel construction of E. coli expression vectors. Methods in Molecular Biology, 1116, 209–234. https://doi.org/10.1007/978-1-62703-764-8_15
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