Effects of chronically exposure to flor and 7,12-dimethylbenzanthracene (dmba) on spermatogenesis and testicular histopathology in Rats

Abstract

This study aimed to investigate the effects of both sodium fluoride (NaF) and polycyclic aromatic hydrocarbon (PAH) derivative such as 7,12-dimethylbenz[a]anthracene (DMBA) singly and in combination on spermatogenesis and testis histopathology in rats. Forty male Wistar albino rats weighing 250–300 g used. On the 90th day of the experiment, intracardiac blood samples and testes were taken. While there was no change in FSH and LH levels in the groups, testosterone levels were significantly decreased in the other groups according to the control group (P<0.001). It was decreased the ratio of motility of sperm, sperm density and, increased the abnormal sperm rate by chronic fluorosis and DMBA toxicity in rats significantly. In histopathological examinations; A decrease in the number of spermatocytes and spermatozoa in the tubulus wall of the testes of rats in the NaF (30 ppm) group was observed. When testicular tissues of rats in the DMBA (10 mg/kg) group were examined, atrophy in tubulus, edema in intertubular spaces and a decrease in the number of spermatocytes in the tubulus wall were found. When testes tissues of rats in the group of NaF (30 ppm) + DMBA (10 mg/kg) were examined, atrophy in seminiferous tubules, severe degenerative and necrotic changes in Spermatogoniums on the walls of tubules and thinning in tubule walls were observed. There is not found spermatozoon in the tubul lumens. Severe edema in the intertubular spaces, dilatation and hyperemia in the veins found in these regions. As a result, it was observed that NaF and DMBA had a negative effect on testicular tissue and fertility singly, and in combination, this effect was further exacerbated.