Transcription activator-like effector nucleases (TALENs) have rapidly developed into a powerful tool for genome editing. To avoid labor-intensive and time-consuming experimental screening for active TALENs, a scoring system can help select optimal target sites. Here we describe a procedure to design active TALENs using a scoring system named Scoring Algorithm for Predicted TALEN Activity (SAPTA) and a method to test the activity of individual and pairs of TALENs. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Lin, Y., Cradick, T. J., & Bao, G. (2014). Designing and testing the activities of TAL effector nucleases. Methods in Molecular Biology, 1114, 203–219. https://doi.org/10.1007/978-1-62703-761-7_13
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