Bacterial chromosomal DNA is packed within a non-membranous structure, the nucleoid, thanks to nucleoid associated proteins (NAPs). The role of bacterial amyloid has recently emerged among these NAPs, particularly with the nucleoid-associated protein Hfq that plays a direct role in DNA compaction. In this chapter, we present a 3D imaging technique, cryo-soft X-ray tomography (cryo-SXT) to obtain a detailed 3D visualization of subcellular bacterial structures, especially the nucleoid. Cryo-SXT imaging of native unlabeled cells enables observation of the nucleoid in 3D with a high resolution, allowing to evidence in vivo the role of amyloids on DNA compaction. The precise experimental methods to obtain 3D tomograms will be presented.
CITATION STYLE
Cossa, A., Wien, F., Turbant, F., Kaczorowski, T., Węgrzyn, G., Arluison, V., … Pereiro, E. (2022). Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo-Soft X-Ray Tomography. In Methods in Molecular Biology (Vol. 2538, pp. 319–333). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2529-3_21
Mendeley helps you to discover research relevant for your work.