Polyacrylamide gel electrophoresis is a widely used technique for RNA analysis and purification. The polyacrylamide matrix is highly versatile for chemical derivitization, enabling facile exploitation of thio-mercury chemistry without the need of tedious manipulations and/or expensive coupling reagents, which often give low yields and side products. Here, we describe the use of [(N-acryloylamino)phenyl]mercuric chloride in three-layered polyacrylamide gels to detect, separate, quantify, and analyze sulfur-containing RNAs. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Biondi, E., & Burke, D. H. (2012). Separating and analyzing sulfur-containing rnas with organomercury gels. Methods in Molecular Biology, 883, 111–120. https://doi.org/10.1007/978-1-61779-839-9_8
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