Pronuclear Transfer in Human Oocytes

Abstract

The method described is for early pronuclear transfer (PNT) in normally fertilized human eggs. The PNT procedure should be performed shortly after the appearance of the two pronuclei. Each pronucleus is pinched off with a minimal amount of surrounding cytoplasm to form a membrane enclosed karyoplast. The karyoplasts are then fused with a fertilized egg which has had its pronuclei removed. An experienced individual will achieve approximately 90% survival of the fertilized eggs manipulated.