High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital readout of mRNA levels over several orders of magnitude, as well as mapping the transcripts to the nucleotide level. Here we describe two different RNA-seq approaches, including one that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5′ end of all Leishmania (and other trypanosomatid) mRNAs.
CITATION STYLE
Myler, P. J., McDonald, J. A., Alcolea, P. J., & Sur, A. (2019). Quantitative RNA Analysis Using RNA-Seq. In Methods in Molecular Biology (Vol. 1971, pp. 95–108). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9210-2_4
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