OLE RNA protects extremophilic bacteria from alcohol toxicity

22Citations
Citations of this article
44Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

OLE (Ornate, Large, Extremophilic) RNAs represent a recently discovered non-coding RNA class found in extremophilic anaerobic bacteria, including certain human pathogens. OLE RNAs exhibit several unusual characteristics that indicate a potentially novel function, including exceptionally high expression and localization to cell membranes via interaction with a protein partner called OLE-associated protein (OAP). In the current study, new genetic and phenotypic characteristics of OLE RNA from Bacillus halodurans C-125 were established. OLE RNA is transcribed at high levels from its own promoter under normal growth conditions and the transcript is exceptionally stable compared to most other RNAs. Expression is increased by ∼7-fold when cells are exposed to near lethal concentrations of short-chain alcohols such as ethanol or methanol. Strains wherein the genes for OLE and/or OAP are deleted are more susceptible to growth inhibition by alcohol and also become more sensitive to cold. Normal growth characteristics can be restored by expressing the genes for OLE and OAP from plasmids or from elsewhere on the chromosome. Our findings confirm a functional link between OLE and OAP and reveal the importance of a large non-coding RNA in the response to alcohol-induced stress. © The Author(s) 2012.

References Powered by Scopus

edgeR: A Bioconductor package for differential expression analysis of digital gene expression data

28028Citations
N/AReaders
Get full text

Mapping and quantifying mammalian transcriptomes by RNA-Seq

10926Citations
N/AReaders
Get full text

SOAP2: An improved ultrafast tool for short read alignment

3094Citations
N/AReaders
Get full text

Cited by Powered by Scopus

RNA-binding proteins in bacteria

162Citations
N/AReaders
Get full text

LncRNAs: emerging players in gene regulation and disease pathogenesis

91Citations
N/AReaders
Get full text

Large noncoding RNAs in bacteria

44Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Wallace, J. G., Zhou, Z., & Breaker, R. R. (2012). OLE RNA protects extremophilic bacteria from alcohol toxicity. Nucleic Acids Research, 40(14), 6898–6907. https://doi.org/10.1093/nar/gks352

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 20

65%

Researcher 8

26%

Professor / Associate Prof. 3

10%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 26

72%

Biochemistry, Genetics and Molecular Bi... 6

17%

Chemistry 3

8%

Engineering 1

3%

Article Metrics

Tooltip
Social Media
Shares, Likes & Comments: 1

Save time finding and organizing research with Mendeley

Sign up for free