Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) is the preferred approach to map histone modifications and identify cis-regulatory DNA elements throughout the genome. Multiple methods have been described to increase the efficiency of library preparation and to reduce hands-on time as well as costs. This review describes detailed steps to perform cell fixation, chromatin shearing, immunoprecipitation, and sequencing library preparation for a batch of 48–96 samples with small cell numbers. The protocol implements a semiautomated platform to reduce technical variability and improve signal-to-noise ratio as well as reduce hands-on time, thus allowing large-scale epigenetic studies of clinical samples with limited cell numbers.
CITATION STYLE
Youhanna Jankeel, D., Cayford, J., Schmiedel, B. J., Vijayanand, P., & Seumois, G. (2018). An integrated and semiautomated microscaled approach to profile Cis-regulatory elements by histone modification ChIP-seq for large-scale epigenetic studies. In Methods in Molecular Biology (Vol. 1799, pp. 303–326). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7896-0_22
Mendeley helps you to discover research relevant for your work.