Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan

Abstract

A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated as NCCP-1340T, was isolated from a hot water spring, Tatta Pani, Pakistan. Cells of strain NCCP-1340Twere observed to be aerobic, rod shaped, catalase and urease positive but H2S production and oxidase negative. Growth was observed at pH 6.0–8.0 (optimum pH 7.0) and at 20–40 °C (optimum 37 °C). The strain could tolerate 0–8 % NaCl (optimum 2 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NCCP-1340Tbelongs to the genus Nocardioides and is closely related to Nocardioides iriomotensis JCM 17985T(96.8 %), Nocardioides daedukensis KCTC 19601T(96.6 %), Nocardioides jensenii KCTC 9134T(96.1 %) and Nocardioides daejeonensis KCTC 19772T(96.1 %). The DNA–DNA relatedness values of strain NCCP-1340Twith N. iriomotensis JCM 17985T, N. daedukensis KCTC 19601Tand N. jensenii KCTC 9134Twere found to be less than 53 %. The DNA G+C content of strain NCCP-1340Twas determined to be 71.8 mol %. The affiliation of strain NCCP-1340Tto the genus Nocardioides was further supported by chemotaxonomic data which showed the presence of MK-8(H4) as major menaquinone system; iso-C16:0, C17:0, C16:010-methyl, iso-C15:0and C 15:0as major cellular fatty acids; and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and unidentified glycolipids and polar lipids in the polar lipids profile. The cell wall peptidoglycan contained LL-diaminopimelic acid as the diagnostic amino acid. On the basis of physiological and biochemical characteristics and the phylogenetic analyses, strain NCCP-1340Tcan be distinguished from the closely related taxa and thus represents a novel species of the genus Nocardioides, for which the name Nocardioides pakistanensis sp. nov. is proposed with the type strain NCCP-1340T(= DSM 29942T = JCM 30630T).