Mutated derivatives of carboxypeptidase yscY (CPY) are potent substrates to study protein quality control and protein degradation in different cell compartments in yeast. Depending on the subcellular compartment of interest, the design of the model substrate used has to be adapted. Here, we describe the derivatives of CPY*used in genetic screens based on a sensitive growth test in order to identify new components of the protein quality control systems in different degradation pathways (see Notes 1-3). © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Stolz, A., & Wolf, D. H. (2012). Use of CPY* and its derivatives to study protein quality control in various cell compartments. Methods in Molecular Biology, 832, 489–504. https://doi.org/10.1007/978-1-61779-474-2_35
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